Photo of Yuguo Lei

Yuguo Lei

Associate Professor of Biomedical Engineering and Graduate Program Coordinator
Faculty Director, Sartorius Cell Culture Facility, Huck Institutes of Life Sciences


  • Biomedical Engineering
  • Huck Institutes of the Life Sciences

515 Chemical and Biomedical Engineering Building


Research Website

Interest Areas:

Cell therapy; Cell manufacturing; Biomaterials




  • B.S., Chemistry, Peking University, 1999
  • M.Phil., Polymer Science, Hong Kong University of Science & Technology, 2002
  • M.S., Molecular and Medical Pharmacology, University of California, Los Angeles, 2006
  • Ph.D., Chemical and Biomolecular Engineering, University of California, Los Angeles, 2010
  • Postdoc, Regenerative Medicine, University of California, Berkeley, 2014


Journal Articles

  • Q Liu, Z Liu, H Gu, Y Ge, X Wu, F Zuo, Q Du, Yuguo Lei, Z Wang and H Lin, 2022, "Comparative study of differentiating human pluripotent stem cells into vascular smooth muscle cells in hydrogel-based culture methods.", Regenerative therapy, 22, pp. 39-49
  • O Wang, L Han, H Lin, M Tian, S Zhang, B Duan, S Chung, C Zhang, Xiaojun Lian, Yong Wang and Yuguo Lei, 2022, "Fabricating 3-dimensional human brown adipose microtissues for transplantation studies.", Bioactive materials, 22, pp. 518-534
  • Qiang Li, Ying Pan, Li Han, Yakun Yang, Xinran Wu and Yuguo Lei, 2022, "Improving three-dimensional human pluripotent cell culture efficiency via surface molecule coating", Front. Chem. Eng., 4
  • Wen Xue, Bo Liu, Haipeng Zhang, Sangjin Ryu, Mitchell Kuss, Devanshi Shukla, Guoku Hu, Wen Shi, Xiping Jiang, Yuguo Lei and others, 2022, "Controllable fabrication of alginate/poly-L-ornithine polyelectrolyte complex hydrogel networks as therapeutic drug and cell carriers", Acta biomaterialia, 138, pp. 182-192
  • Li Han, Carlos P Jara, Ou Wang, Yu Shi, Xinran Wu, Sandra Thibivilliers, W\'oycicki, Rafa\l K, Mark A Carlson, William H Velander, Ara\'ujo, Eliana P and others, 2022, "Isolating and cryopreserving pig skin cells for single-cell RNA sequencing study", PloS one, 17, (2), pp. e0263869
  • Zhanqi Wang, Fuxing Zuo, Qing Liu, Xuesheng Wu, Qian Du, Yuguo Lei, Zhangmin Wu and Haishuang Lin, 2021, "Comparative Study of Human Pluripotent Stem Cell-Derived Endothelial Cells in Hydrogel-Based Culture Systems", ACS omega, 6, (10), pp. 6942--6952
  • Carlos Poblete Jara, Carolina Motter Catarino, Yuguo Lei, Velloso, L\'\icio Augusto, Pankaj Karande, William H Velander and Eliana Pereira de Araujo, 2021, "Demonstration of re-epithelialization in a bioprinted human skin equivalent wound model", Bioprinting, 24, pp. e00102
  • Ou Wang, Li Han, Haishuang Lin, Mingmei Tian, Shuyang Zhang, Bin Duan, Soonkyu Chung, Chi Zhang, Xiaojun Lian, Yuguo Lei and others, 2021, "Engineered human brown adipocyte microtissues improved glucose and insulin homeostasis in high fat diet-induced obese and diabetic mice", bioRxiv
  • Bo Liu, Yunfan Kong, Wen Shi, Mitchell Kuss, Ke Liao, Guoku Hu, Peng Xiao, Jagadesan Sankarasubramanian, Chittibabu Guda, Xinglong Wang and others, 2021, "Exosomes derived from differentiated human ADMSC with the Schwann cell phenotype modulate peripheral nerve-related cellular functions", Bioactive Materials
  • Yuguo Lei, Stephanie Michelle Willerth and Tiago G Fernandes, 2021, "Stem Cell Systems Bioengineering", Frontiers in Bioengineering and Biotechnology, 9
  • Amy Lingel, Haishuang Lin, Yuval Gavriel, Eric Weaver, Pascal Polepole, Virginia Lopez, Yuguo Lei, Thomas M Petro, Beka Solomon, Chi Zhang and others, 2020, "Amyloid precursor protein is a restriction factor that protects against Zika virus infection in mammalian brains", Journal of Biological Chemistry, 295, (50), pp. 17114--17127
  • Carlos Poblete Jara, Ou Wang, Thais Paulino do Prado, Ayman Ismail, Frank Marco Fabian, Han Li, Licio A Velloso, Mark A Carlson, William Burgess, Yuguo Lei and others, 2020, "Novel fibrin-fibronectin matrix accelerates mice skin wound healing", Bioactive Materials, 5, (4), pp. 949--962
  • Frank M Fabian, Ayman EA Ismail, Ou Wang, Yuguo Lei and William H Velander, 2020, "Reversible associations between human plasma fibronectin and fibrinogen $\gamma$$\gamma$’heterodimer observed by high pressure size exclusion chromatography and dynamic light scattering", Analytical Biochemistry, 598, pp. 113701
  • Kan Liu, Chi Zhang, Soonkyu Chung, Bin Duan and Yuguo Lei, 2019, "A Scalable and Efficient Bioprocess for Manufacturing Human Pluripotent Stem Cell-Derived Endothelial Cells", Stem Cell Reports, 11, pp. 454--469
  • Yuguo Lei, Qiang Li and Haishuang Lin, 2019, "A scalable and physiologically relevant system for human induced pluripotent stem cell expansion and differentiation"
  • Haishuang Lin, Qian Du, Qiang Li, Ou Wang, Zhanqi Wang, Kan Liu, Leonard Akert, Chi Zhang, Soonkyu Chung, Bin Duan and others, 2019, "Differentiating human pluripotent stem cells into vascular smooth muscle cells in three dimensional thermoreversible hydrogels", Biomaterials science, 7, (1), pp. 347--361
  • Haishuang Lin, Xuefeng Qiu, Qian Du, Qiang Li, Ou Wang, Leonard Akert, Zhanqi Wang, Dirk Anderson, Kan Liu, Linxia Gu and others, 2019, "Engineered microenvironment for manufacturing human pluripotent stem cell-derived vascular smooth muscle cells", Stem Cell Reports, 12, (1), pp. 84--97
  • Haishuang Lin, Qiang Li, Qian Du, Ou Wang, Zhanqi Wang, Leonard Akert, Mark A Carlson, Chi Zhang, Anuradha Subramanian, Matthew Lunning and others, 2019, "Integrated generation of induced pluripotent stem cells in a low-cost device", Biomaterials, 189, pp. 23--36
  • Haishuang Lin, Qian Du, Qiang Li, Ou Wang, Zhanqi Wang, Christian Elowsky, Kan Liu, Chi Zhang, Soonkyu Chung, Bin Duan and others, 2019, "Manufacturing human pluripotent stem cell derived endothelial cells in scalable and cell-friendly microenvironments", Biomaterials science, 7, (1), pp. 373--388
  • Hozhabr Mozafari, Lulu Wang, Yuguo Lei and Linxia Gu, 2019, "Multi-scale modeling of the lamellar unit of arterial media", Nanotechnology Reviews, 8, (1), pp. 539--547
  • Qiang Li, Qiaofeng Wang, Ou Wang, Kaifeng Shao, Haishuang Lin and Yuguo Lei, 2018, "A simple and scalable hydrogel-based system for culturing protein-producing cells", PloS one, 13, (1), pp. e0190364
  • Ou Wang, Ayman Ismail, Frank Marco Fabian, Haishuang Lin, Qiang Li, Christian Elowsky, Mark A Carlson, Wilson Burgess, William H Velander, Srivatsan Kidambi and others, 2018, "A totally recombinant fibrin matrix for mesenchymal stem cell culture and delivery", Journal of Biomedical Materials Research Part A, 106, (12), pp. 3135--3142
  • Haishuang Lin, Qiang Li, Ou Wang, Jack Rauch, Braden Harm, Hendrik J Viljoen, Chi Zhang, Erika Van Wyk, Chi Zhang and Yuguo Lei, 2018, "Automated expansion of primary human T cells in scalable and cell-friendly hydrogel microtubes for adoptive immunotherapy", Advanced healthcare materials, 7, (15), pp. 1701297
  • Mitchell Kuss, Jiyoung Kim, Dianjun Qi, Shaohua Wu, Yuguo Lei, Soonkyu Chung and Bin Duan, 2018, "Effects of tunable, 3D-bioprinted hydrogels on human brown adipocyte behavior and metabolic function", Acta biomaterialia, 71, pp. 486--495
  • Dianjun Qi, Shaohua Wu, Haishuang Lin, Mitchell A Kuss, Yuguo Lei, Alexey Krasnoslobodtsev, Shaheen Ahmed, Chi Zhang, Hyung Joon Kim, Peng Jiang and others, 2018, "Establishment of a human iPSC-and nanofiber-based microphysiological blood--brain barrier system", ACS applied materials \& interfaces, 10, (26), pp. 21825--21835
  • Haishuang Lin, Qian Du, Qiang Li, Ou Wang, Zhanqi Wang, Kan Liu, Christian Elowsky, Chi Zhang and Yuguo Lei, 2018, "Hydrogel-based bioprocess for scalable manufacturing of human pluripotent stem cell-derived neural stem cells", ACS applied materials \& interfaces, 10, (35), pp. 29238--29250
  • Qiang Li, Haishuang Lin, Jack Rauch, Loic P Deleyrolle, Brent A Reynolds, Hendrik J Viljoen, Chi Zhang, Linxia Gu, Erika Van Wyk and Yuguo Lei, 2018, "Scalable culturing of primary human glioblastoma tumor-initiating cells with a cell-friendly culture system", Scientific reports, 8, (1), pp. 1--13
  • Ayman EA Ismail, Frank M Fabian, Ou Wang, Yuguo Lei, Mark A Carlson, Wilson H Burgess and William H Velander, 2018, "The isolation of a plasma-derived $\gamma$$\gamma$’fibrinogen: Fibronectin mixture that forms a novel polymeric matrix", Process Biochemistry, 75, pp. 257--265
  • Barbara L Ekerdt, Christina M Fuentes, Yuguo Lei, Maroof M Adil, Anusuya Ramasubramanian, Rachel A Segalman and David V Schaffer, 2018, "Thermoreversible Hyaluronic Acid-PNIPAAm Hydrogel Systems for 3D Stem Cell Culture", Advanced healthcare materials, 7, (12), pp. 1800225
  • Haishuang Lin, Qiang Li and Yuguo Lei, 2017, "An integrated miniature bioprocessing for personalized human induced pluripotent stem cell expansion and differentiation into neural stem cells", Scientific reports, 7, (1), pp. 1--13
  • Haishuang Lin, Qiang Li and Yuguo Lei, 2017, "Three-dimensional tissues using human pluripotent stem cell spheroids as biofabrication building blocks", Biofabrication, 9, (2), pp. 025007
  • Qiang Li, Haishuang Lin, Ou Wang, Xuefeng Qiu, Srivatsan Kidambi, Loic P Deleyrolle, Brent A Reynolds and Yuguo Lei, 2016, "Scalable production of glioblastoma tumor-initiating cells in 3 dimension thermoreversible hydrogels", Scientific reports, 6, (1), pp. 1--10
  • Yuguo Lei, Daeun Jeong, Jifang Xiao and David V Schaffer, 2014, "Developing defined and scalable 3D culture systems for culturing human pluripotent stem cells at high densities", Cellular and molecular bioengineering, 7, (2), pp. 172--183
  • Yuguo Lei and David V Schaffer, 2013, "A fully defined and scalable 3D culture system for human pluripotent stem cell expansion and differentiation", Proceedings of the National Academy of Sciences, 110, (52), pp. E5039--E5048
  • Talar Tokatlian, Cynthia Cam, Shayne N Siegman, Yuguo Lei and Tatiana Segura, 2012, "Design and characterization of microporous hyaluronic acid hydrogels for in vitro gene transfer to mMSCs", Acta biomaterialia, 8, (11), pp. 3921--3931
  • Yuguo Lei, Maha Rahim, Quinn Ng and Tatiana Segura, 2011, "Hyaluronic acid and fibrin hydrogels with concentrated DNA/PEI polyplexes for local gene delivery", Journal of controlled release, 153, (3), pp. 255--261
  • Jianjun Zhang, Yuguo Lei, Anandika Dhaliwal, Quinn KT Ng, Juanjuan Du, Ming Yan, Yunfeng Lu and Tatiana Segura, 2011, "Protein- polymer nanoparticles for nonviral gene delivery", Biomacromolecules, 12, (4), pp. 1006--1014
  • Yuguo Lei, Shiva Gojgini, Jonathan Lam and Tatiana Segura, 2011, "The spreading, migration and proliferation of mouse mesenchymal stem cells cultured inside hyaluronic acid hydrogels", Biomaterials, 32, (1), pp. 39--47
  • Yuguo Lei, Suxian Huang, Pooria Sharif-Kashani, Yong Chen, Pirouz Kavehpour and Tatiana Segura, 2010, "Incorporation of active DNA/cationic polymer polyplexes into hydrogel scaffolds", Biomaterials, 31, (34), pp. 9106--9116
  • Yuguo Lei, Quinn KT Ng and Tatiana Segura, 2010, "Two and three-dimensional gene transfer from enzymatically degradable hydrogel scaffolds", Microscopy research and technique, 73, (9), pp. 910--917
  • Yuguo Lei and Tatiana Segura, 2009, "DNA delivery from matrix metalloproteinase degradable poly (ethylene glycol) hydrogels to mouse cloned mesenchymal stem cells", Biomaterials, 30, (2), pp. 254--265
  • Lu-Tao Weng, Kai-Mo Ng, Zhuo Lin Cheung, Yuguo Lei and Chi-Ming Chan, 2006, "Quantitative analysis of styrene-pentafluorostyrene random copolymers by ToF-SIMS and XPS", Surface and Interface Analysis: An International Journal devoted to the development and application of techniques for the analysis of surfaces, interfaces and thin films, 38, (1), pp. 32--43
  • Yuchun Ou, Yuguo Lei, Xiaoping Fang and Guisheng Yang, 2004, "Maleic anhydride grafted thermoplastic elastomer as an interfacial modifier for polypropylene/polyamide 6 blends", Journal of applied polymer science, 91, (3), pp. 1806--1815
  • Yu-Guo Lei, Chi-Ming Chan, Yong Wang, Kai-Mo Ng, Yong Jiang and Li Lin, 2003, "Growth process of homogeneously and heterogeneously nucleated spherulites as observed by atomic force microscopy", Polymer, 44, (16), pp. 4673--4679
  • Yu-Guo Lei, Zhuo-Lin Cheung, Kai-Mo Ng, Lin Li, Lu-Tao Weng and Chi-Ming Chan, 2003, "Surface chemical and morphological properties of a blend containing semi-crystalline and amorphous polymers studied with ToF-SIMS, XPS and AFM", Polymer, 44, (14), pp. 3883--3890
  • Yu-Guo Lei, Kai-Mo Ng, Lu-Tao Weng, Chi-Ming Chan and Lin Li, 2003, "XPS C 1s binding energies for fluorocarbon--hydrocarbon microblock copolymers", Surface and Interface Analysis: An International Journal devoted to the development and application of techniques for the analysis of surfaces, interfaces and thin films, 35, (10), pp. 852--855
  • Yanhong Luo, Yong Jiang, Xigao Jin, Lin Li, Yuguo Lei and Chiming Chan, 2002, "Recent progresses of polymer crystallization studied by AFM", Chinese Science Bulletin, 47, (21), pp. 1761--1765
  • Yong Jiang, Qun Gu, Lin Li, De-Yan Shen, Xi-Gao Jin, Yu-Guo Lei and Chi-Ming Chan, 2002, "Structural changes during isothermal crystallization of a poly (bisphenol A-co-decane ether) polymer", Polymer, 43, (21), pp. 5615--5621
  • Yu-Guo Lei, Chi-Ming Chan, Jian-Xiong Li, Kai-Mo Ng, Yong Wang, Yong Jiang and Lin Li, 2002, "The birth of an embryo and development of the founding lamella of spherulites as observed by atomic force microscopy", Macromolecules, 35, (18), pp. 6751--6753
  • Lin Li, Chi-Ming Chan, Kai-Mo Ng, Yuguo Lei and Lu-Tao Weng, 2001, "A time-of-ight secondary ion mass spectrometry study of sequential polymers with a well-de\textregistered ned segmental length", Polymer, 42, (6841), pp. 6849
  • Lin Li, Chi-Ming Chan, King Lun Yeung, Jian-Xiong Li, Kai-Mo Ng and Yuguo Lei, 2001, "Direct observation of growth of lamellae and spherulites of a semicrystalline polymer by AFM", Macromolecules, 34, (2), pp. 316--325

Conference Proceedings

  • Zhuo Lin Cheung, Yuguo Lei, Kai Mo Ng, Lin Li, Lutao Weng and Chi Ming Chan, 2004, "Surface Chemical and Morphological Properties of a Blend Containing Semi-Crystalline and Amorphous Components Compared with a Polymer Containing the same Crystalline and Amorphous Components"


  • Yuguo Lei, S M Willerth and T G Fernandes, 2021, "Editorial: Stem Cell Systems Bioengineering.", Frontiers in bioengineering and biotechnology, 9, pp. 693107

Research Projects

Honors and Awards


Service to Penn State:

Service to External Organizations:




The Department of Biomedical Engineering administers the undergraduate major in biomedical engineering, and is a part of the university-wide Intercollege Graduate Degree Program, offering both M.S. and Ph.D. degrees in Bioengineering. Our work combines traditional engineering principles with medicine and technology for the betterment of human health and society. 

Department of Biomedical Engineering

122 Chemical and Biomedical Engineering Building

The Pennsylvania State University

University Park, PA 16802-4400

Phone: 814-863-6614