Photo of Xiaojun Lian

Xiaojun Lian

Associate Professor of Biomedical Engineering


  • Biomedical Engineering

W342 Millennium Science Complex


Personal or Departmental Website

Research Areas:

Cell and Molecular Bioengineering




  • BS, Bioengineering, Zhejiang University, 2006
  • Ph D, Chemical Engineering, University of Wisconsin - Madison, 2012


Journal Articles

  • Reza Nouri, Yuqian Jiang, Xiaojun Lian and Weihua Guan, 2020, "Sequence-Specific Recognition of HIV-1 DNA with Solid-State CRISPR-Cas12a-Assisted Nanopores (SCAN).", ACS sensors, 5, (5), pp. 1273-1280
  • N Zhao, J Coyne, L Abune, P Shi, Xiaojun Lian, Ge Zhang and Yong Wang, 2020, "Exogenous Signaling Molecules Released from Aptamer-Functionalized Hydrogels Promote the Survival of Mesenchymal Stem Cell Spheroids.", ACS applied materials & interfaces
  • G B Kim, V Aragon-Sanabria, Lauren Randolph, H Jiang, J A Reynolds, B S Webb, Achuthamangalam Madhankumar, Xiaojun Lian, James R Connor, Jian Yang and Cheng Dong, 2020, "High-affinity mutant Interleukin-13 targeted CAR T cells enhance delivery of clickable biodegradable fluorescent nanoparticles to glioblastoma.", Bioactive materials, 5, (3), pp. 624-635
  • Y Chang, P B Hellwarth, Lauren N Randolph, Yufei Sun, Y Xing, Wuqiang Zhu, Xiaojun Lian and Xiaoping Bao, 2020, "Fluorescent indicators for continuous and lineage-specific reporting of cell-cycle phases in human pluripotent stem cells.", Biotechnology and bioengineering
  • Yuqian Jiang and Xiaojun Lian, 2020, "Heart regeneration with human pluripotent stem cells: Prospects and challenges.", Bioactive materials, 5, (1), pp. 74-81
  • Lauren N Randolph, Xiaoping Bao, Michael Oddo and Xiaojun Lian, 2019, "Sex-dependent VEGF expression underlies variations in human pluripotent stem cell to endothelial progenitor differentiation.", Scientific reports, 9, (1), pp. 16696
  • Lauren Randolph and Xiaojun Lian, 2019, "Beyond Purple Hearts: A Colorful Approach to Isolate Distinct Heart Cells from Human iPSCs.", Cell Stem Cell
  • Lauren Nicole Randolph, Agamoni Bhattacharyya and Xiaojun Lance Lian, 2019, "Human Beta Cells Generated from Pluripotent Stem Cells or Cellular Reprogramming for Curing Diabetes", Regenerative Engineering and Translational Medicine
  • Yuqian Jiang, Yuxiao Zhou, Xiaoping Bao, Chuanxin Chen, Lauren Randolph, Jing Du and Xiaojun Lance Lian, 2018, "An ultrasensitive calcium reporter system via CRISPR-Cas9 mediated genome editing in human pluripotent stem cells", iScience
  • Kylie Foo, Miia Lehtinen, Chuen Yan Leung, Xiaojun Lian, Jiejia Xu, Wendy Keung, Lin Geng, Terje Kolstad, Sebastian Thams, Andy Wong, Nicodemus Wong, Kristine Bylund, Chikai Zhou, Xiaobing He, Shao-Bo Jin, Jonathan Clarke, Urban Lendahl, Ronald Li, William Louch and Kenneth Chien, 2018, "Human ISL1+ Ventricular Progenitors Self-Assemble into an In Vivo Functional Heart Patch and Preserve Cardiac Function After Infarction", Molecular Therapy, 26, (7), pp. 1644-1659
  • Lauren Randolph, Yuqian Jiang and Xiaojun Lian, 2017, "Stem Cell Engineering and Differentiation for Disease Modeling and Cell-based Therapies", AIMS Cell and Tissue Engineering, 1, (2), pp. 140-157
  • Xiaoping Bao, Xiaojun Lian, T Qian, V J Bhute, T Han and Sean P Palecek, 2017, "Directed differentiation and long-term maintenance of epicardial cells derived from human pluripotent stem cells under fully defined conditions.", Nature protocols, 12, (9), pp. 1890-1900
  • X Bao, V J Bhute, T Han, T Qian, Xiaojun Lian and S P Palecek, 2017, "Human pluripotent stem cell-derived epicardial progenitors can differentiate to endocardial-like endothelial cells.", Bioengineering & translational medicine, 2, (2), pp. 191-201
  • Lauren N Randolph, X Bao, Chikai Zhou and Xiaojun Lian, 2017, "An all-in-one, Tet-On 3G inducible PiggyBac system for human pluripotent stem cells and derivatives", Scientific Reports, 7, (1), pp. 1549
  • Xiaoping Bao, Xiaojun Lian, T Hacker, E Schmuck, T Qian, V Bhute, T Han, M Shi, L Drowley, A Plowright, Q Wang, M Goumans and S Palecek, 2016, "Long-term self-renewing human epicardial cells generated from pluripotent stem cells under defined xeno-free conditions", Nature Biomedical Engineering, 1, pp. 0003
  • Xiaojun Lian, Jiejia Xu, Xiaoping Bao and Lauren Randolph, 2016, "Interrogating Canonical Wnt Signaling Pathway in Human Pluripotent Stem Cell Fate Decisions Using CRISPR-Cas9", Cellular and molecular bioengineering, 9, (3), pp. 325–334
  • E B Braune, Y L Tsoi, Y P Phoon, S Landor, H Silva Cascales, D Ramsköld, Q Deng, A Lindqvist, Xiaojun Lian, C Sahlgren, S B Jin and U Lendahl, 2016, "Loss of CSL Unlocks a Hypoxic Response and Enhanced Tumor Growth Potential in Breast Cancer Cells.", Stem Cell Reports, 6, (5), pp. 643-651
  • B S Soh, S Y Ng, H Wu, K Buac, J H Park, Xiaojun Lian, J Xu, K S Foo, U Felldin, X He, M Nichane, H Yang, L Bu, R A Li, B Lim and K R Chien, 2016, "Endothelin-1 supports clonal derivation and expansion of cardiovascular progenitors derived from human embryonic stem cells.", Nature communications, 7, pp. 10774
  • C Hsiao, M Lampe, S Nillasithanukroh, W Han, Xiaojun Lian and S P Palecek, 2016, "Human pluripotent stem cell culture density modulates YAP signaling.", Biotechnology Journal, 11, (5), pp. 662-75
  • X Bao, Xiaojun Lian, K K Dunn, M Shi, T Han, T Qian, V J Bhute, S G Canfield and S P Palecek, 2015, "Chemically-defined albumin-free differentiation of human pluripotent stem cells to endothelial progenitor cells.", Stem cell research, 15, (1), pp. 122-9
  • Xiaojun Lian, X Bao, M Zilberter, M Westman, A Fisahn, C Hsiao, L B Hazeltine, K K Dunn, T J Kamp and S P Palecek, 2015, "Chemically defined, albumin-free human cardiomyocyte generation.", Nature Methods, 12, (7), pp. 595-6
  • Xiaojun Lian, X Bao, A Al-Ahmad, J Liu, Y Wu, W Dong, K K Dunn, E V Shusta and S P Palecek, 2014, "Efficient differentiation of human pluripotent stem cells to endothelial progenitors via small-molecule activation of WNT signaling.", Stem Cell Reports, 3, (5), pp. 804-16
  • L B Hazeltine, M G Badur, Xiaojun Lian, A Das, W Han and S P Palecek, 2014, "Temporal impact of substrate mechanics on differentiation of human embryonic stem cells to cardiomyocytes.", Acta biomaterialia, 10, (2), pp. 604-12
  • Xiaojun Lian and K R Chien, 2013, "The years of the monkey.", Cell research, 23, (10), pp. 1161-2
  • Xiaojun Lian, J Zhang, K Zhu, T J Kamp and S P Palecek, 2013, "Insulin inhibits cardiac mesoderm, not mesendoderm, formation during cardiac differentiation of human pluripotent stem cells and modulation of canonical Wnt signaling can rescue this inhibition.", Stem cells, 31, (3), pp. 447-57
  • Xiaojun Lian, J Selekman, X Bao, C Hsiao, K Zhu and S P Palecek, 2013, "A small molecule inhibitor of SRC family kinases promotes simple epithelial differentiation of human pluripotent stem cells.", PLOS ONE, 8, (3), pp. e60016
  • Xiaojun Lian, J Zhang, S M Azarin, K Zhu, L B Hazeltine, X Bao, C Hsiao, T J Kamp and S P Palecek, 2013, "Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/ß-catenin signaling under fully defined conditions.", Nature protocols, 8, (1), pp. 162-75
  • J Zhang, M Klos, G F Wilson, A M Herman, Xiaojun Lian, K K Raval, M R Barron, L Hou, A G Soerens, J Yu, S P Palecek, G E Lyons, J A Thomson, T J Herron, J Jalife and T J Kamp, 2012, "Extracellular matrix promotes highly efficient cardiac differentiation of human pluripotent stem cells: the matrix sandwich method.", Circulation research, 111, (9), pp. 1125-36
  • Xiaojun Lian, C Hsiao, G Wilson, K Zhu, L B Hazeltine, S M Azarin, K K Raval, J Zhang, T J Kamp and S P Palecek, 2012, "Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling.", Proceedings of the National Academy of Sciences of the United States of America, 109, (27), pp. E1848-57
  • G Ghosh, Xiaojun Lian, S J Kron and S P Palecek, 2012, "Properties of resistant cells generated from lung cancer cell lines treated with EGFR inhibitors.", BMC cancer, 12, pp. 95
  • S M Azarin, Xiaojun Lian, E A Larson, H M Popelka, J J de Pablo and S P Palecek, 2012, "Modulation of Wnt/ß-catenin signaling in human embryonic stem cells using a 3-D microwell array.", Biomaterials, 33, (7), pp. 2041-9
  • L B Hazeltine, C S Simmons, M R Salick, Xiaojun Lian, M G Badur, W Han, S M Delgado, T Wakatsuki, W C Crone, B L Pruitt and S P Palecek, 2012, "Effects of substrate mechanics on contractility of cardiomyocytes generated from human pluripotent stem cells.", International journal of cell biology, 2012, pp. 508294

Research Projects

Honors and Awards

  • CAREER Award, National Science Foundation, 2020 - 2025
  • Trailblazer Award from National Institute of Biomedical Imaging and Bioengineering, NIH, March 2018 - February 2021
  • 2018 Junior Investigator Award of Advanced Biomanufacturing, Biomedical Engineering Society, June 2018
  • 2017 Junior Investigator Award of Advanced Biomanufacturing, Biomedical Engineering Society, August 2017
  • Young Innovator Award, Cellular and Molecular Bioengineering Journal/BMES, October 2016
  • The Cozzarelli Prize of the National Academy of Science, National Academy of Science, May 2013
  • Top Poster Prize & Best Poster Presentation, Society for Biological Engineering, 2012
  • Ronald A. Ragatz Outstanding Teaching Assistant Award, University of Wisconsin - Madison, 2009
  • Chu Kochen Scholarship, the highest academic award of Zhejiang University, Zhejiang University, 2006
  • Rising Star Award from Cellular and Molecular Bioengineering SIG, BMES, Biomedical Engineering Society, January 2020


Service to Penn State:

  • Committee Work, Member, Engineering Faculty Council member, Penn State, Engineering Faculty Council, May 2020
  • Contribution to Programs to Enhance Equal Opportunity/Cultural Diversity, Other, Interviewer, Interview candidates for Millennium Scholars Program, February 2019 - February 2019
  • Other, Advisor, Perform mock graduate school interviews, MILLENNIUM SCHOLARS Program, October 2017 - October 2017
  • Other, Presenter, Contribution to setting up a new center for regenerative and genetic medicine, November 2016 - November 2016

Service to External Organizations:

  • Other, Panelist, Member for NIH Cardiac Contractility, Hypertrophy, and Failure (CCHF) Study Section, June 2020 - June 2020
  • Other, Chairperson, Section Chair of BMES Annual Meeting Stem Cell Engineering Section, October 2019 - October 2019
  • Other, BMES Advanced Biomanufacturing Special Interest Group, August 2018
  • Organizing Conferences and Service on Conference Committees, Organizer, BMES Advanced Biomanufacturing Special Interest Group, March 2018
  • Other, Reviewer, 2017 - 2017



The Department of Biomedical Engineering administers the undergraduate major in biomedical engineering, and is a part of the university-wide Intercollege Graduate Degree Program, offering both M.S. and Ph.D. degrees in Bioengineering. Our work combines traditional engineering principles with medicine and technology for the betterment of human health and society. 

Department of Biomedical Engineering

122 Chemical and Biomedical Engineering Building

The Pennsylvania State University

University Park, PA 16802-4400

Phone: 814-863-6614