Research

Undergraduate Students

Mobirise

Cardiac Differentiation

We are implementing a variety of techniques, including small molecule mediated hPSC differentiation and CRISPR-Cas9 reprogramming, to efficiently generate cells of the cardiac lineages for regenerative medicine and therapeutic development.

Recent Publications

Xiaoping Bao, Xiaojun Lian, Tongcheng Qian, Vijesh J. Bhute, Tianxiao Han, Sean P. Palecek. Directed differentiation and long-term maintenance of epicardial cells derived from human pluripotent stem cells under fully defined conditions, Nature Protocols (2017) Sep;12(9):1890-1900. doi: 10.1038/nprot.2017.080.

Jiang, Y. et al. An ultrasensitive calcium reporter system via CRISPR-Cas9 mediated genome editing in human pluripotent stem cells. ISCIENCE 9, 27–35 (2018)

Mobirise

Beta Cell Differentiation

Our lab is using both directed differentiation and genetic reprogramming techniques to generate functional beta cells from human embryonic stem cells for diabetes therapy.

Recent Publications

Lauren Nicole Randolph, Agamoni Bhattacharyya and Xiaojun Lance Lian, 2018, "Human Beta Cells Generated from Pluripotent Stem Cells or Cellular Reprogramming for Curing Diabetes", Regenerative Engineering and Translational Medicine

Mobirise

Universal Donor Cells

Our lab is working to develop universal donor cells for cardiac transplantation medicine applications and creating new molecular biology tools to enable this work.

Recent Publications

Lauren Randolph, Xiaoping Bao, Chikai Zhou, Xiaojun Lian. An all-in-one, Tet-On 3G inducible PiggyBac system for human pluripotent stem cells and derivatives, Scientific Reports 7 (2017), Article number: 1549 doi:10.1038/s41598-017-01684-6

Publications

2022

43. Jung J, Chang Y, Jin G, Lian X, Bao X. Temporal Expression of Transcription Factor ID2 Improves Natural Killer Cell Differentiation from Human Pluripotent Stem Cells. ACS Synth Biol. 2022 Jun 17

42. Chang Y, Syahirah R, Wang X, Jin G, Torregrosa-Allen S, Elzey BD, Hummel SN, Wang T, Li C, Lian X, Deng Q, Broxmeyer HE, Bao X. Engineering chimeric antigen receptor neutrophils from human pluripotent stem cells for targeted cancer immunotherapy. Cell Rep. 2022 Jul 19

41. Wang O, Han L, Lin H, Tian M, Zhang S, Duan B, Chung S, Zhang C, Lian X, Wang Y, Lei Y. Fabricating 3-dimensional human brown adipose microtissues for transplantation studies. Bioact Mater. 2022 Oct 27

40. Liang PY, Chang Y, Jin G, Lian X, Bao X. Wnt signaling directs human pluripotent stem cells into vascularized cardiac organoids with chamber-like structures. Front Bioeng Biotechnol. 2022 Nov 18

39. Jung J, Chang Y, Jin G, Deng Q, Lian XL, Bao X. Chemically defined generation of human definitive hematopoietic stem and progenitor cells. STAR Protoc. 2022 Dec 16;4(1):101953.

38. Tahir Haideri, Alessandro Howells, Yuqian Jiang, Jian Yang, Xiaoping Bao, Xiaojun Lance Lian, Robust genome editing via modRNA-based Cas9 or base editor in human pluripotent stem cells, Cell Reports Methods, Volume 2, Issue 9, 2022, 100290, ISSN 2667-2375

2021

37. Yun Chang, Ramizah Syahirah, Stephanie N. Oprescu, Xuepeng Wang, Juhyung Jung, Scott H. Cooper, Sandra Torregrosa-Allen, Bennett D. Elzey, Alan Y. Hsu, Lauren N. Randolph, Yufei Sun, Shihuan Kuang, Hal E. Broxmeyer, Qing Deng, Xiaojun Lian, Xiaoping Bao, Chemically-defined generation of human hemogenic endothelium and definitive hematopoietic progenitor cells, Biomaterials, Volume 285, 2022, 121569, ISSN 0142-9612.

36. Yuqian Jiang, Rachel Catherine Hoenisch, Yun Chang, Xiaoping Bao, Craig E. Cameron, Xiaojun Lance Lian, Robust genome and RNA editing via CRISPR nucleases in PiggyBac systems, Bioactive Materials, Volume 14, 2022, Pages 313-320, ISSN 2452-199X.

35. Jia Zhu, Shangbin Liu, Zhihui Hu, Xianzhe Zhang, Ning Yi, Kairui Tang, Michael Gregory Dexheimer, Xiaojun Lian, Qing Wang, Jian Yang, Jennifer Gray, Huanyu Cheng, Laser-induced graphene non-enzymatic glucose sensors for on-body measurements, Biosensors and Bioelectronics, Volume 193, 2021, 113606, ISSN 0956-5663

34. Reza Nouri, Yuqian Jiang, Zifan Tang, Xiaojun Lance Lian, and Weihua Guan. Detection of SARS-CoV-2 with Solid-State CRISPR-Cas12a-Assisted Nanopores. Nano Letters 2021 21 (19), 8393-8400. DOI: 10.1021/acs.nanolett.1c02974

33. Yuqian Jiang, Chuanxin Chen, Lauren N. Randolph, Songtao Ye, Xin Zhang, Xiaoping Bao, Xiaojun Lance Lian, Generation of pancreatic progenitors from human pluripotent stem cells by small molecules, Stem Cell Reports, Volume 16, Issue 9, 2021

32. Hellwarth, PB, Chang, Y, Das, A, et al. Optogenetic-mediated cardiovascular differentiation and patterning of human pluripotent stem cells. Advanced Genetics. 2021

31. Jiang, Y., Bao, X. & Lian, X.L. A dual cardiomyocyte reporter model derived from human pluripotent stem cells. Stem Cell Res Ther 12, 305 (2021).

2020
30. Kim G.B., Aragon-Sanabria V., Randolph L.N., Jiang H, Reynolds J.A., Webb B.S., Madhankumar A., Lian X., Connor J.R., Yang J., Dong C. High-affinity mutant Interleukin-13 targeted CAR T cells enhance delivery of clickable biodegradable fluorescent nanoparticles to glioblastoma. Bioactive Materials, 5, 3, 624-35 (2020).

29. Chang Y., Hellwarth P.B., Randolph L.N., Sun Y., Xing Y., Zhu W., Lian X.L., Bao X. Fluorescent indicators for continuous and lineage-specific reporting of cell-cycle phases in human pluripotent stem cells. Biotechnology and Bioengineering, 117, 7, 2177-86 (2020).

28. Zhao N., Coyne J., Abune L., Shi P., Lian X.L., Zhang G., Wang Y. Exogenous signaling molecules released from aptamer-functionalized hydrogels promote the survival of mesenchymal stem cell spheroids. ACS Applied Materials and Interfaces, 12, 22, 24599-24610 (2020).

27. Nouri R., Jiang Y., Lian X.L., Guan W. Sequence-specific recognition of HIV-1 DNA with solid-state CRISPR-Cas12a-assisted nanopores (SCAN). ACS Sensors, 5,5, 1273-80 (2020).

2019
26. Randolph LN, Bao X, Oddo M, Lian X. Sex-dependent VEGF expression underlies variations in human pluripotent stem cell to endothelial progenitor differentiation. Scientific Reports (2019).

2018
25. Jiang Y, Zhou Y, Bao X, Chen C, Randolph LN, Du J, Lian X. An ultrasensitive calcium reporter system via CRISPR-Cas9 mediated genome editing in human pluripotent stem cells. ISCIENCE 9, 27–35 (2018).

24. Randolph LN, Bhattacharyya A, and Lian X, 2018, "Human Beta Cells Generated from Pluripotent Stem Cells or Cellular Reprogramming for Curing Diabetes", Regenerative Engineering and Translational Medicine

23. Foo KS, Lehtinen ML, Leung CY, Lian X, Xu J, Keung W, Geng L, Kolstad TRS, Thams S, Wong AO, Wong N Bylund K, Zhou C, He X, Jin SB, Clarke J, Lendhal U, Li RA, Louch WE, Chien KR. Mol Ther. 2018 Jul 5;26(7):1644-1659.

2017
22. Randolph LN, Jiang Y, Lian X. Stem Cell Engineering and Differentiation for Disease Modeling and Cell-based Therapies, AIMS Cell and Tissue Engineering, (2017), 1(2): 140-157. doi: 10.3934/celltissue.2017.2.140.

21. Bao X, Lian X, Qian T, Bhute VJ, Han T, Palecek SP. Directed differentiation and long-term maintenance of epicardial cells derived from human pluripotent stem cells under fully defined conditions, Nature Protocols (2017) Sep;12(9):1890-1900. doi: 10.1038/nprot.2017.080.

20. Bao X, Bhute VJ, Han T, Qian T, Lian X, Palecek SP. Human pluripotent stem cell-derived epicardial progenitors can differentiate to endocardial-like endothelial cells, Bioengineering & Translational Medicine (2017)

19. Randolph LN, Bao X, Zhou C, XiaLian. An all-in-one, Tet-On 3G inducible PiggyBac system for human pluripotent stem cells and derivatives, Scientific Reports 7 (2017), Article number: 1549 doi:10.1038/s41598-017-01684-6

2016
18. Bao X, Lian X, Hacker TA, Schmuck EG, Qian T, Bhute VJ, Han T, Shi M, Drowley L, Plowright A, Wang QD, Goumans MJ, Palecek SP. Long-term self-renewing human epicardial cells generated from pluripotent stem cells under defined xeno-free conditions, Nature Biomedical Engineering 1, 0003 (2016)

17. Lian X, Xu J, Bao X and Randolph LN. Interrogating Canonical Wnt Signaling Pathway in Human Pluripotent Stem Cell Fate Decisions Using CRISPR-Cas9, Cellular and Molecular Bioengineering. 9 (3): 325-334 (2016)

16. Braune E, Tsoi YL, Phoon YP, Landor S, Cascales HS, Ramsköld D, Deng Q, Lindqvist A, Lian X, Sahlgren C, Jin S, Lendahl U. Loss of CSL Unlocks a Hypoxic Response and Enhanced Tumor Growth Potential in Breast Cancer Cells. Stem Cell Reports. 6 (5): 643–651 (2016)

15. Seng Soh B, Ng SY, Wu H, Buac K, Park JC, Lian X, Xu J, Foo KS, Felldin U, He X, Nichane M, Yang H, Bu L, Li RA, Lim B, Chien K. Endothelin-1 supports clonal derivation and expansion of cardiovascular progenitors derived from human embryonic stem cells. Nature Communications. 7: 10774 (2016)

14. Hsiao C, Lampe M, Nillasithanukroh S, Han W, Lian X, Palecek SP. Human pluripotent stem cell culture density modulates YAP signaling. Biotechnol J. 11(5):662-75 (2016)

13. Bao X, Lian X, Palecek SP. Directed Endothelial Progenitor Differentiation from Human Pluripotent Stem Cells Via Wnt Activation Under Defined Conditions. Methods Mol Biol. 1481:183-96 (2016)

2015
12. Lian X, Bao X, Zilberter M, et al. “Chemically defined, albumin-free human cardiomyocyte generation”, Nature Methods 12, 595–596 (2015)

11. Bao X, Lian X, et al. “Chemically-defined albumin-free differentiation of human pluripotent stem cells to endothelial progenitor cells”, Stem Cell Research, 15(1):122-129 (2015) *co-first author

2014
10. Lian X, Bao X, Al-Ahmad A, Liu J, Wu Y, Dong W, Dunn KK, Shusta EV, Palecek SP. “Efficient Differentiation of Human Pluripotent Stem Cells to Endothelial Progenitors via Small-Molecule Activation of WNT Signaling”, Stem Cell Reports 3(5): 804–816 (2014)

9. Lian X, Xu J, Li J, Chien KR. “Next Generation Models of Human Cardiogenesis via Genome Editing”, Cold Spring Harb Perspect Med (2014)

8. Hazeltine LB, Badur MG, Lian X, Das A, Han W, Palecek SP. “Temporal impact of substrate mechanics on differentiation of human embryonic stem cells to cardiomyocytes”, Acta Biomater 10: 604-612 (2014)

2013
7. Lian X, Zhang J, Azarin SM, Zhu K, Hazeltine LB, Bao X, Hsiao C, Kamp TJ, Palecek SP. “Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/beta-catenin signaling under fully defined conditions”, Nature Protocols 8: 162-175 (2013)

6. Lian X, Selekman J, Bao X, Hsiao C, Zhu K, Palecek SP. “A Small Molecule Inhibitor of Src Family Kinases Promotes Simple Epithelial Differentiation of Human Pluripotent Stem Cells”, PLoS ONE 8(3): e60016 (2013)

5. Lian X, Zhang J, Zhu K, Kamp TJ, Palecek SP. “Insulin inhibits cardiac mesoderm, not mesendoderm, formation during cardiac differentiation of human pluripotent stem cells and modulation of canonical Wnt signaling can rescue this inhibition”, Stem Cells 31: 447-457 (2013)

2012
4. Lian X, Hsiao C, Wilson G, Zhu K, Hazeltine LB, Azarin SM, Raval KK, Zhang J, Kamp TJ, Palecek SP. “Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling”, Proc Natl Acad Sci USA 109: E1848-1857 (2012)

3. Zhang J, Klos M, Wilson GF, Herman AM, Lian X, et al. “Extracellular matrix promotes highly efficient cardiac differentiation of human pluripotent stem cells: the matrix sandwich method”, Circ Res 111: 1125-1136 (2012)

2. Hazeltine LB, Simmons CS, Salick MR, Lian X, et al. “Effects of substrate mechanics on contractility of cardiomyocytes generated from human pluripotent stem cells”, Int J Cell Biol 2012: 508294 (2012)

1. Azarin SM, Lian X, Larson EA, Popelka HM, de Pablo JJ, Palecek SP. “Modulation of Wnt/beta-catenin signaling in human embryonic stem cells using a 3-D microwell array”, Biomaterials 33: 2041-2049 (2012)

Mobirise

Address

W342 Millenium Science Complex
University Park, PA 16802

Contacts

Email: lian@psu.edu
Phone: (814) 865-8093

Links

BME Department
Biology Department

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