Undergraduate Students


Cardiac Differentiation

We are implementing a variety of techniques, including small molecule mediated hPSC differentiation and CRISPR-Cas9 reprogramming, to efficiently generate cells of the cardiac lineages for regenerative medicine and therapeutic development.

Recent Publications

Xiaoping Bao, Xiaojun Lian, Tongcheng Qian, Vijesh J. Bhute, Tianxiao Han, Sean P. Palecek. Directed differentiation and long-term maintenance of epicardial cells derived from human pluripotent stem cells under fully defined conditions, Nature Protocols (2017) Sep;12(9):1890-1900. doi: 10.1038/nprot.2017.080.

Jiang, Y. et al. An ultrasensitive calcium reporter system via CRISPR-Cas9 mediated genome editing in human pluripotent stem cells. ISCIENCE 9, 27–35 (2018)


Beta Cell Differentiation

Our lab is using both directed differentiation and genetic reprogramming techniques to generate functional beta cells from human embryonic stem cells for diabetes therapy.

Recent Publications

Lauren Nicole Randolph, Agamoni Bhattacharyya and Xiaojun Lance Lian, 2018, "Human Beta Cells Generated from Pluripotent Stem Cells or Cellular Reprogramming for Curing Diabetes", Regenerative Engineering and Translational Medicine


Universal Donor Cells

Our lab is working to develop universal donor cells for cardiac transplantation medicine applications and creating new molecular biology tools to enable this work.

Recent Publications

Lauren Randolph, Xiaoping Bao, Chikai Zhou, Xiaojun Lian. An all-in-one, Tet-On 3G inducible PiggyBac system for human pluripotent stem cells and derivatives, Scientific Reports 7 (2017), Article number: 1549 doi:10.1038/s41598-017-01684-6



38. Tahir Haideri, Alessandro Howells, Yuqian Jiang, Jian Yang, Xiaoping Bao, Xiaojun Lance Lian, Robust genome editing via modRNA-based Cas9 or base editor in human pluripotent stem cells, Cell Reports Methods, Volume 2, Issue 9, 2022, 100290, ISSN 2667-2375


37. Yun Chang, Ramizah Syahirah, Stephanie N. Oprescu, Xuepeng Wang, Juhyung Jung, Scott H. Cooper, Sandra Torregrosa-Allen, Bennett D. Elzey, Alan Y. Hsu, Lauren N. Randolph, Yufei Sun, Shihuan Kuang, Hal E. Broxmeyer, Qing Deng, Xiaojun Lian, Xiaoping Bao, Chemically-defined generation of human hemogenic endothelium and definitive hematopoietic progenitor cells, Biomaterials, Volume 285, 2022, 121569, ISSN 0142-9612.

36. Yuqian Jiang, Rachel Catherine Hoenisch, Yun Chang, Xiaoping Bao, Craig E. Cameron, Xiaojun Lance Lian, Robust genome and RNA editing via CRISPR nucleases in PiggyBac systems, Bioactive Materials, Volume 14, 2022, Pages 313-320, ISSN 2452-199X.

35. Jia Zhu, Shangbin Liu, Zhihui Hu, Xianzhe Zhang, Ning Yi, Kairui Tang, Michael Gregory Dexheimer, Xiaojun Lian, Qing Wang, Jian Yang, Jennifer Gray, Huanyu Cheng, Laser-induced graphene non-enzymatic glucose sensors for on-body measurements, Biosensors and Bioelectronics, Volume 193, 2021, 113606, ISSN 0956-5663

34. Reza Nouri, Yuqian Jiang, Zifan Tang, Xiaojun Lance Lian, and Weihua Guan. Detection of SARS-CoV-2 with Solid-State CRISPR-Cas12a-Assisted Nanopores. Nano Letters 2021 21 (19), 8393-8400. DOI: 10.1021/acs.nanolett.1c02974

33. Yuqian Jiang, Chuanxin Chen, Lauren N. Randolph, Songtao Ye, Xin Zhang, Xiaoping Bao, Xiaojun Lance Lian, Generation of pancreatic progenitors from human pluripotent stem cells by small molecules, Stem Cell Reports, Volume 16, Issue 9, 2021

32. Hellwarth, PB, Chang, Y, Das, A, et al. Optogenetic-mediated cardiovascular differentiation and patterning of human pluripotent stem cells. Advanced Genetics. 2021

31. Jiang, Y., Bao, X. & Lian, X.L. A dual cardiomyocyte reporter model derived from human pluripotent stem cells. Stem Cell Res Ther 12, 305 (2021).

30. Kim G.B., Aragon-Sanabria V., Randolph L.N., Jiang H, Reynolds J.A., Webb B.S., Madhankumar A., Lian X., Connor J.R., Yang J., Dong C. High-affinity mutant Interleukin-13 targeted CAR T cells enhance delivery of clickable biodegradable fluorescent nanoparticles to glioblastoma. Bioactive Materials, 5, 3, 624-35 (2020).

29. Chang Y., Hellwarth P.B., Randolph L.N., Sun Y., Xing Y., Zhu W., Lian X.L., Bao X. Fluorescent indicators for continuous and lineage-specific reporting of cell-cycle phases in human pluripotent stem cells. Biotechnology and Bioengineering, 117, 7, 2177-86 (2020).

28. Zhao N., Coyne J., Abune L., Shi P., Lian X.L., Zhang G., Wang Y. Exogenous signaling molecules released from aptamer-functionalized hydrogels promote the survival of mesenchymal stem cell spheroids. ACS Applied Materials and Interfaces, 12, 22, 24599-24610 (2020).

27. Nouri R., Jiang Y., Lian X.L., Guan W. Sequence-specific recognition of HIV-1 DNA with solid-state CRISPR-Cas12a-assisted nanopores (SCAN). ACS Sensors, 5,5, 1273-80 (2020).

26. Randolph LN, Bao X, Oddo M, Lian X. Sex-dependent VEGF expression underlies variations in human pluripotent stem cell to endothelial progenitor differentiation. Scientific Reports (2019).


23. Foo KS, Lehtinen ML, Leung CY, Lian X, Xu J, Keung W, Geng L, Kolstad TRS, Thams S, Wong AO, Wong N Bylund K, Zhou C, He X, Jin SB, Clarke J, Lendhal U, Li RA, Louch WE, Chien KR. Mol Ther. 2018 Jul 5;26(7):1644-1659.








W342 Millenium Science Complex
University Park, PA 16802 


Email: lian@psu.edu
Phone: (814) 865-8093                    

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