We are implementing a variety of techniques, including small molecule mediated hPSC differentiation and CRISPR-Cas9 reprogramming, to efficiently generate cells of the cardiac lineages for regenerative medicine and therapeutic development.
Xiaoping Bao, Xiaojun Lian, Tongcheng Qian, Vijesh J. Bhute, Tianxiao Han, Sean P. Palecek. Directed differentiation and long-term maintenance of epicardial cells derived from human pluripotent stem cells under fully defined conditions, Nature Protocols (2017) Sep;12(9):1890-1900. doi: 10.1038/nprot.2017.080.
Jiang, Y. et al. An ultrasensitive calcium reporter system via CRISPR-Cas9 mediated genome editing in human pluripotent stem cells. ISCIENCE 9, 27–35 (2018)
Our lab is using both directed differentiation and genetic reprogramming techniques to generate functional beta cells from human embryonic stem cells for diabetes therapy.
Lauren Nicole Randolph, Agamoni Bhattacharyya and Xiaojun Lance Lian, 2018, "Human Beta Cells Generated from Pluripotent Stem Cells or Cellular Reprogramming for Curing Diabetes", Regenerative Engineering and Translational Medicine
Our lab is working to develop universal donor cells for cardiac transplantation medicine applications and creating new molecular biology tools to enable this work.
Lauren Randolph, Xiaoping Bao, Chikai Zhou, Xiaojun Lian. An all-in-one, Tet-On 3G inducible PiggyBac system for human pluripotent stem cells and derivatives, Scientific Reports 7 (2017), Article number: 1549 doi:10.1038/s41598-017-01684-6