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Undergraduate Students


Cardiac Differentiation

We are implementing a variety of techniques, including small molecule mediated hPSC differentiation and CRISPR-Cas9 reprogramming, to efficiently generate cells of the cardiac lineages for regenerative medicine and therapeutic development.

Recent Publications

Xiaoping Bao, Xiaojun Lian, Tongcheng Qian, Vijesh J. Bhute, Tianxiao Han, Sean P. Palecek. Directed differentiation and long-term maintenance of epicardial cells derived from human pluripotent stem cells under fully defined conditions, Nature Protocols (2017) Sep;12(9):1890-1900. doi: 10.1038/nprot.2017.080.

Jiang, Y. et al. An ultrasensitive calcium reporter system via CRISPR-Cas9 mediated genome editing in human pluripotent stem cells. ISCIENCE 9, 27–35 (2018)


Beta Cell Differentiation

Our lab is using both directed differentiation and genetic reprogramming techniques to generate functional beta cells from human embryonic stem cells for diabetes therapy.

Recent Publications

Lauren Nicole Randolph, Agamoni Bhattacharyya and Xiaojun Lance Lian, 2018, "Human Beta Cells Generated from Pluripotent Stem Cells or Cellular Reprogramming for Curing Diabetes", Regenerative Engineering and Translational Medicine


Universal Donor Cells

Our lab is working to develop universal donor cells for cardiac transplantation medicine applications and creating new molecular biology tools to enable this work.

Recent Publications

Lauren Randolph, Xiaoping Bao, Chikai Zhou, Xiaojun Lian. An all-in-one, Tet-On 3G inducible PiggyBac system for human pluripotent stem cells and derivatives, Scientific Reports 7 (2017), Article number: 1549 doi:10.1038/s41598-017-01684-6


33. Yuqian Jiang, Chuanxin Chen, Lauren N. Randolph, Songtao Ye, Xin Zhang, Xiaoping Bao, Xiaojun Lance Lian, Generation of pancreatic progenitors from human pluripotent stem cells by small molecules, Stem Cell Reports, Volume 16, Issue 9, 2021.

32. Hellwarth, PB, Chang, Y, Das, A, et al. Optogenetic-mediated cardiovascular differentiation and patterning of human pluripotent stem cells. Advanced Genetics. 2021.

31. Jiang, Y., Bao, X. & Lian, X.L. A dual cardiomyocyte reporter model derived from human pluripotent stem cells. Stem Cell Res Ther 12, 305 (2021).

30. Kim G.B., Aragon-Sanabria V., Randolph L.N., Jiang H, Reynolds J.A., Webb B.S., Madhankumar A., Lian X., Connor J.R., Yang J., Dong C. High-affinity mutant Interleukin-13 targeted CAR T cells enhance delivery of clickable biodegradable fluorescent nanoparticles to glioblastoma. Bioactive Materials, 5, 3, 624-35 (2020).

29. Chang Y., Hellwarth P.B., Randolph L.N., Sun Y., Xing Y., Zhu W., Lian X.L., Bao X. Fluorescent indicators for continuous and lineage-specific reporting of cell-cycle phases in human pluripotent stem cells. Biotechnology and Bioengineering, 117, 7, 2177-86 (2020).

28. Zhao N., Coyne J., Abune L., Shi P., Lian X.L., Zhang G., Wang Y. Exogenous signaling molecules released from aptamer-functionalized hydrogels promote the survival of mesenchymal stem cell spheroids. ACS Applied Materials and Interfaces, 12, 22, 24599-24610 (2020).

27. Nouri R., Jiang Y., Lian X.L., Guan W. Sequence-specific recognition of HIV-1 DNA with solid-state CRISPR-Cas12a-assisted nanopores (SCAN). ACS Sensors, 5,5, 1273-80 (2020).

26. Randolph LN, Bao X, Oddo M, Lian X. Sex-dependent VEGF expression underlies variations in human pluripotent stem cell to endothelial progenitor differentiation. Scientific Reports (2019).


23. Foo KS, Lehtinen ML, Leung CY, Lian X, Xu J, Keung W, Geng L, Kolstad TRS, Thams S, Wong AO, Wong N Bylund K, Zhou C, He X, Jin SB, Clarke J, Lendhal U, Li RA, Louch WE, Chien KR. Mol Ther. 2018 Jul 5;26(7):1644-1659.








W342 Millenium Science Complex
University Park, PA 16802 


Email: lian@psu.edu
Phone: (814) 865-8093